Morphofunctional analysis of the interaction between human keratinocyte and metal particles of dental implant alloys: in vitro study
admin-cespu
Principal Investigator:
Orlanda Torres, Fernando Ferreira, Françoise Kleinsorgena, Erica Moreno de Albuquerque, Oscar Carvalho
Leader Institution:
UNIPRO, IUCS-CESPU
Research Team:
Júlio C. M. Souza,
Funding entity:
CESPU
Budget:
3800 €
Period covered:
01/09/2025-2026
Abstract:
This project aims to investigate, in an in vitro model, the cellular response associated with the release of metallic particles resulting from the corrosion of alloys used in dental implants. Three alloys widely used in clinical practice will be analyzed: Ti6Al4V, commercially pure titanium (grade IV) and Ti15Zr. Cylindrical samples will be immersed in modified artificial saliva and evaluated at 3, 6, and 12 months, with surface characterization by scanning field emission electron microscopy (FEG-SEM), optical profilometry and X-ray scattering spectroscopy (EDS). At the same time, the generated metallic particles will be used to expose human keratinocytes and evaluate at 1, 3 and 7 days, cell viability (MTT assay), morphological changes and internalization of nanoparticles. The proposed hypothesis is that the released metal debris promotes an epithelial inflammatory response, potentially contributing to the long-term failure of dental implants.
This project aims to investigate, in an in vitro model, the cellular response associated with the release of metallic particles resulting from the corrosion of alloys used in dental implants. Three alloys widely used in clinical practice will be analyzed: Ti6Al4V, commercially pure titanium (grade IV) and Ti15Zr. Cylindrical samples will be immersed in modified artificial saliva and evaluated at 3, 6, and 12 months, with surface characterization by scanning field emission electron microscopy (FEG-SEM), optical profilometry and X-ray scattering spectroscopy (EDS). At the same time, the generated metallic particles will be used to expose human keratinocytes and evaluate at 1, 3 and 7 days, cell viability (MTT assay), morphological changes and internalization of nanoparticles. The proposed hypothesis is that the released metal debris promotes an epithelial inflammatory response, potentially contributing to the long-term failure of dental implants.